Sugarcane with altered sugar metabolism & new promotor elements (SARSI) / SARSI
Objection To The Application By Sasri In Respect Of A Trial With Gm Sugarcane, To The National Department Of Agriculture, South Africa.
African Centre for Biosafety, June 2005
Application by SASRI and Available Information
An application has been made by the South African Sugarcane Research Institute (SASRI) to conduct a trial with genetically modified sugarcane (The North Coast Courier May 13 2005). According to the public notice, several independent modifications, falling into two categories, are being investigated:
1. To determine sucrose yields in mature plants: endogenous enzymes (phosphofructokinase, aldolase, fructose 2,6-bisphosphate) of sugarcane plants of cultivars 88H0019, N19, N27 and N31, NCo310, have been up- or downregulated. For the purposes of the following discussion, these are hereinafter collectively referred to as T1 modifications.
2. To establish whether promoter elements isolated from sugarcane can be used to target gene expression to specific regions of the plant: sugarcane plants, cultivar NCo310, have been transformed with novel promoter elements and a reporter gene b-glucuronidase (GUS) from E.coli. For the purposes of the following discussion, these are hereinafter collectively referred to as T2 modifications